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Development of surface plasmon oscillations as a probe of macromolecular adsorption
Abstract
The development of devices and techniques for making in-situ measurements of macromolecular adsorption from solution onto metal films using surface plasmon oscillations (SPOs) as a probe is described. Research underpinning this work is reviewed from an historical perspective in order to clarify misconceptions encountered in the literature. Derivation of equations used to describe macromolecular adsorption in terms of SPO phenomena is described from first principles. Two generations of devices have been designed and constructed which allow real time measurements of adsorption to be made for times ranging from 10 seconds to weeks. Devices and techniques developed for this research are used to measure adsorption in immunological protein and synthetic polymer systems. Use of SPO techniques for an immunoassay method is demonstrated. The methods developed allow collection of data for quantitative analysis of adsorption kinetics. The adsorption kinetics manifest concentration and time dependence. These dependencies are examined on time scales from several seconds to several hours. Theoretical predictions by some authors for the behavior of the adsorption kinetics is compared with the data.
Subject Area
Physics|Biophysics|Optics
Recommended Citation
Mayo, Charles Steven, "Development of surface plasmon oscillations as a probe of macromolecular adsorption" (1991). Doctoral Dissertations Available from Proquest. AAI9120915.
https://scholarworks.umass.edu/dissertations/AAI9120915