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Author ORCID Identifier
https://orcid.org/0000-0001-7496-0416
AccessType
Open Access Dissertation
Document Type
dissertation
Degree Name
Doctor of Philosophy (PhD)
Degree Program
Food Science
Year Degree Awarded
2019
Month Degree Awarded
September
First Advisor
Lili He
Subject Categories
Agriculture | Food Science | Microbiology
Abstract
The growth curves for E. coli O157:H7 (#043888) are reported. We make the case that the onset of stationary growth is the optimal point at which a bacteria culture is considered suitable for quantitative Raman analyses. The optimal conditions for 3-mercaptophenylboronic acid coating of bacteria cells is also reported. Fundamental drawbacks of the status-quo approach have been elucidated and overcome, based on measurable improvements to the experimental methodology. This approach is shown to be suitable for the evaluation of bacterial rinse-washing efficacy by means of Raman light-scattering. The data were compared to label-free applications and the measurable differences between each approach were defined. Future use of 3-mercaptophenylboronic acid labelling for SERS analyses of bacteria should strictly follow the methods that we outlined within this paper. A real-time method to surveil mass bacterial communities directly in situ is also reported. The approach was successfully employed to indiscriminately monitor mass bacteria populations directly among plant tissue.
DOI
https://doi.org/10.7275/15177318
Recommended Citation
Hickey, Michael, "Surface-enhanced Raman Spectroscopic Imaging of Bacteria within Fresh Produce In Situ" (2019). Doctoral Dissertations. 1677.
https://doi.org/10.7275/15177318
https://scholarworks.umass.edu/dissertations_2/1677
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.