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Imaging and detecting intercellular tensile forces in spheroids and embryoid bodies using lipid-modified DNA probes

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dc.contributor.authorTian, Qian
dc.contributor.authorYang, Feiyu
dc.contributor.authorJiang, Han
dc.contributor.authorBhattacharyya, Priyanka
dc.contributor.authorXie, Tianfa
dc.contributor.authorAli, Ahsan Ausaf
dc.contributor.authorSun, Yubing
dc.contributor.authorYou, Mingxu
dc.date2023-11-27T15:17:17.000
dc.date.accessioned2024-04-26T09:01:30Z
dc.date.available2023-11-27T00:00:00Z
dc.date.issued2023-01-01
dc.description.abstractCells continuously experience and respond to different physical forces that are used to regulate their physiology and functions. Our ability to measure these mechanical cues is essential for understanding the bases of various mechanosensing and mechanotransduction processes. While multiple strategies have been developed to study mechanical forces within two-dimensional (2D) cell culture monolayers, the force measurement at cell-cell junctions in real three-dimensional (3D) cell models is still pretty rare. Considering that in real biological systems, cells are exposed to forces from 3D directions, measuring these molecular forces in their native environment is thus highly critical for the better understanding of different development and disease processes. We have recently developed a type of DNA-based molecular probe for measuring intercellular tensile forces in 2D cell models. Herein, we will report the further development and first-time usage of these molecular tension probes to visualize and detect mechanical forces within 3D spheroids and embryoid bodies (EBs). These probes can spontaneously anchor onto live cell membranes via the attached lipid moieties. By varying the concentrations of these DNA probes and their incubation time, we have first characterized the kinetics and efficiency of probe penetration and loading onto tumor spheroids and stem cell EBs of different sizes. After optimization, we have further imaged and measured E-cadherin-mediated forces in these 3D spheroids and EBs for the first time. Our results indicated that these DNA-based molecular tension probes can be used to study the spatiotemporal distributions of target mechanotransduction processes. These powerful imaging tools may be potentially applied to fill the gap between ongoing research of biomechanics in 2D systems and that in real 3D cell complexes.
dc.identifier.doihttps://doi.org/10.3389/fcell.2023.1220079
dc.identifier.urihttps://hdl.handle.net/20.500.14394/6667
dc.relation.ispartofFrontiers in Cell and Developmental Biology
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=2761&context=chem_faculty_pubs&unstamped=1
dc.rightsUMass Amherst Open Access Policy
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.issue11
dc.source.statuspublished
dc.subjectcell-cell junction
dc.subjectDNA probes
dc.subjectfluorescence imaging
dc.subjectmechanotransduction
dc.subjecttensile forces
dc.subject3D cell model
dc.titleImaging and detecting intercellular tensile forces in spheroids and embryoid bodies using lipid-modified DNA probes
dc.typearticle
dc.typearticle
digcom.contributor.authorTian, Qian
digcom.contributor.authorYang, Feiyu
digcom.contributor.authorJiang, Han
digcom.contributor.authorBhattacharyya, Priyanka
digcom.contributor.authorXie, Tianfa
digcom.contributor.authorAli, Ahsan Ausaf
digcom.contributor.authorSun, Yubing
digcom.contributor.authorYou, Mingxu
digcom.date.embargo2023-11-27T00:00:00-08:00
digcom.identifierchem_faculty_pubs/1493
digcom.identifier.contextkey36339375
digcom.identifier.submissionpathchem_faculty_pubs/1493
dspace.entity.typePublication
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