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Chemogenomic profiling: Identifying the functional interactions of small molecules in yeast

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dc.contributor.authorFlaherty, Patrick
dc.date2023-09-23T16:43:15.000
dc.date.accessioned2024-04-26T18:39:19Z
dc.date.available2024-04-26T18:39:19Z
dc.date.issued2004-01-01
dc.description.abstractWe demonstrate the efficacy of a genome-wide protocol in yeast that allows the identification of those gene products that functionally interact with small molecules and result in the inhibition of cellular proliferation. Here we present results from screening 10 diverse compounds in 80 genome-wide experiments against the complete collection of heterozygous yeast deletion strains. These compounds include anticancer and antifungal agents, statins, alverine citrate, and dyclonine. In several cases, we identified previously known interactions; furthermore, in each case, our analysis revealed novel cellular interactions, even when the relationship between a compound and its cellular target had been well established. In addition, we identified a chemical core structure shared among three therapeutically distinct compounds that inhibit the ERG24 heterozygous deletion strain, demonstrating that cells may respond similarly to compounds of related structure. The ability to identify on-and-off target effects in vivo is fundamental to understanding the cellular response to small-molecule perturbants.
dc.identifier.urihttps://hdl.handle.net/20.500.14394/34327
dc.relation.ispartofProceedings of the National Academy of Sciences
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=2271&context=math_faculty_pubs&unstamped=1
dc.relation.urlhttps://works.bepress.com/patrick-flaherty/2/download/
dc.source.beginpage793
dc.source.endpage798
dc.source.issue3
dc.source.issue101
dc.source.statuspublished
dc.subjectprofiling
dc.subjectgenetics
dc.subjectmolecules
dc.subjectyeast
dc.subjectBiomedical Engineering and Bioengineering
dc.subjectEngineering
dc.titleChemogenomic profiling: Identifying the functional interactions of small molecules in yeast
dc.typearticle
dc.typearticle
digcom.contributor.authorisAuthorOfPublication|email:flaherty@math.umass.edu|Flaherty, Patrick
digcom.identifiermath_faculty_pubs/1272
digcom.identifier.contextkey9464911
digcom.identifier.submissionpathmath_faculty_pubs/1272
dspace.entity.typePublication
relation.isAuthorOfPublicationa213aa05-9a0c-453e-abc1-41e72fb4bdaa
relation.isAuthorOfPublication.latestForDiscoverya213aa05-9a0c-453e-abc1-41e72fb4bdaa
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