Publication:
A Metagenomic Approach to Evaluating Surface Water Quality in Haiti

dc.contributor.authorRoy, Monika A.
dc.contributor.authorArnaud, Jean M.
dc.contributor.authorJasmin, Paul M.
dc.contributor.authorHamner, Steve
dc.contributor.authorHasan, Nur A.
dc.contributor.authorColwell, Rita R.
dc.contributor.authorFord, Timothy E.
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentEquipes mobiles d’intervention rapide (EMIRA) du Ministère de la Santé Publique et de la Population (MSPP)
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentUniversity of Maryland
dc.contributor.departmentUniversity of Maryland
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.date2023-09-24T01:08:03.000
dc.date.accessioned2024-04-26T16:35:19Z
dc.date.available2019-10-21T00:00:00Z
dc.date.issued2018-01-01
dc.description.abstractThe cholera epidemic that occurred in Haiti post-earthquake in 2010 has resulted in over 9000 deaths during the past eight years. Currently, morbidity and mortality rates for cholera have declined, but cholera cases still occur on a daily basis. One continuing issue is an inability to accurately predict and identify when cholera outbreaks might occur. To explore this surveillance gap, a metagenomic approach employing environmental samples was taken. In this study, surface water samples were collected at two time points from several sites near the original epicenter of the cholera outbreak in the Central Plateau of Haiti. These samples underwent whole genome sequencing and subsequent metagenomic analysis to characterize the microbial community of bacteria, fungi, protists, and viruses, and to identify antibiotic resistance and virulence associated genes. Replicates from sites were analyzed by principle components analysis, and distinct genomic profiles were obtained for each site. Cholera toxin converting phage was detected at one site, and Shiga toxin converting phages at several sites. Members of the Acinetobacter family were frequently detected in samples, including members implicated in waterborne diseases. These results indicate a metagenomic approach to evaluating water samples can be useful for source tracking and the surveillance of pathogens such as Vibrio cholerae over time, as well as for monitoring virulence factors such as cholera toxin.
dc.identifier.doihttps://doi.org/10.3390/ijerph15102211
dc.identifier.urihttps://hdl.handle.net/20.500.14394/22928
dc.relation.ispartofInternational Journal of Environmental Research amd Public Health
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1012&context=ehs_faculty_pubs&unstamped=1
dc.rightsUMass Amherst Open Access Policy
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.issue10
dc.source.issue15
dc.source.statuspublished
dc.subjectcholera
dc.subjectmetagenomic analysis
dc.subjectbioinformatics
dc.subjectwhole genome sequencing; principle components analysis
dc.subjectwater quality
dc.subjectenvironmental sampling
dc.subjectHaiti
dc.subjectOther Medicine and Health Sciences
dc.titleA Metagenomic Approach to Evaluating Surface Water Quality in Haiti
dc.typearticle
dc.typearticle
digcom.contributor.authorRoy, Monika A.
digcom.contributor.authorArnaud, Jean M.
digcom.contributor.authorJasmin, Paul M.
digcom.contributor.authorHamner, Steve
digcom.contributor.authorHasan, Nur A.
digcom.contributor.authorColwell, Rita R.
digcom.contributor.authorFord, Timothy E.
digcom.date.embargo2019-10-21T00:00:00-07:00
digcom.identifierehs_faculty_pubs/13
digcom.identifier.contextkey15589674
digcom.identifier.submissionpathehs_faculty_pubs/13
dspace.entity.typePublication
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