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Cell Modulation Using Functionalized Nanoparticles

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dc.contributor.advisorVincent M. Rotello
dc.contributor.authorTang, Rui
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.date2023-09-23T15:12:32.000
dc.date.accessioned2024-04-26T16:15:50Z
dc.date.available2024-04-26T16:15:50Z
dc.date.submittedSeptember
dc.date.submitted2016
dc.description.abstractMonolayer functionalized ultra-small gold nanoparticles (AuNPs) provide a versatile platform for applications in cell research. Through rational design of surface ligands, the chemistry of AuNPs are precisely regulated at atomic level. In this dissertation, applications of AuNPs in cell modulation are discussed. The topics are split into two categories. In the first category, functionalized AuNPs are harnessed to generate a robust monolayer on cell culture surface for cell modulation. The proliferation and behavior of different types of cancer cells and normal cells are modulated by tuning the surface ligands of AuNPs. Fate decision of mesenchymal stem cells are also modulated using the same strategy. In the second category, AuNPs are assembled to nanoparticle stabilized capsules (NPSCs) for the delivery of a variety of proteins to cytosol of cells. Using this method, phenotype of cells are rapidly switched without genomic disturbance. In addition, subcellular localization of proteins are also controlled by the combination of subcellular localization signals and NPSC delivery platform. The first non-peptide synthetic nuclear localization signal based on boronate is discovered using NPSC delivery platform as well.
dc.description.degreeDoctor of Philosophy (PhD)
dc.description.departmentChemistry
dc.identifier.doihttps://doi.org/10.7275/8542389.0
dc.identifier.orcidN/A
dc.identifier.urihttps://hdl.handle.net/20.500.14394/20018
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1742&context=dissertations_2&unstamped=1
dc.source.statuspublished
dc.subjectcell modulation
dc.subjectnanoparticle
dc.subjectnanocapsule
dc.subjectstem cell
dc.subjectprotein delivery
dc.subjectnucleus localization
dc.subjectBiology and Biomimetic Materials
dc.subjectMaterials Chemistry
dc.subjectOther Chemistry
dc.titleCell Modulation Using Functionalized Nanoparticles
dc.typeopenaccess
dc.typearticle
dc.typedissertation
digcom.contributor.authorisAuthorOfPublication|email:bio.nanoparticle@gmail.com|institution:University of Massachusetts Amherst|Tang, Rui
digcom.identifierdissertations_2/769
digcom.identifier.contextkey8542389
digcom.identifier.submissionpathdissertations_2/769
dspace.entity.typePublication
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