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Inhibitory Effects of Peptide Lunasin in Colorectal Cancer HCT-116 Cells and Their Tumorsphere-Derived Subpopulation

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dc.contributor.authorFernández-Tomé, Samuel
dc.contributor.authorXu, Fei
dc.contributor.authorHan, Yanhui
dc.contributor.authorHernández-Ledesma, Bianca
dc.contributor.authorXiao, Hang
dc.contributor.departmentInstituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM CEI UAM+CSIC
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentInstituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM CEI UAM+CSIC)
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.date2023-09-24T07:23:15.000
dc.date.accessioned2024-04-26T17:31:15Z
dc.date.available2021-10-05T00:00:00Z
dc.date.issued2020-01-01
dc.description.abstractThe involvement of cancer stem-like cells (CSC) in the tumor pathogenesis has profound implications for cancer therapy and chemoprevention. Lunasin is a bioactive peptide from soybean and other vegetal sources with proven protective activities against cancer and other chronic diseases. The present study focused on the cytotoxic effect of peptide lunasin in colorectal cancer HCT-116 cells, both the bulk tumor and the CSC subpopulations. Lunasin inhibited the proliferation and the tumorsphere-forming capacity of HCT-116 cells. Flow cytometry results demonstrated that the inhibitory effects were related to apoptosis induction and cell cycle-arrest at G1 phase. Moreover, lunasin caused an increase in the sub-GO/G1 phase of bulk tumor cells, linked to the apoptotic events found. Immunoblotting analysis further showed that lunasin induced apoptosis through activation of caspase-3 and cleavage of PARP, and could modulate cell cycle progress through the cyclin-dependent kinase inhibitor p21. Together, these results provide new evidence on the chemopreventive activity of peptide lunasin on colorectal cancer by modulating both the parental and the tumorsphere-derived subsets of HCT-116 cells.
dc.identifier.doihttps://doi.org/10.3390/ijms21020537
dc.identifier.urihttps://hdl.handle.net/20.500.14394/29591
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1036&context=foodsci_faculty_pubs&unstamped=1
dc.rightsUMass Amherst Open Access Policy
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.issue2
dc.source.issuePeptides for Health Benefits 2019
dc.source.issue21
dc.source.statuspublished
dc.subjectcolorectal cancer
dc.subjectcancer stem cells
dc.subjectchemoprevention
dc.subjectbioactive peptide
dc.subjectlunasin
dc.titleInhibitory Effects of Peptide Lunasin in Colorectal Cancer HCT-116 Cells and Their Tumorsphere-Derived Subpopulation
dc.typearticle
dc.typearticle
digcom.contributor.authorisAuthorOfPublication|email:fernandeztome.samuel@gmail.com|institution:Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM CEI UAM+CSIC|Fernández-Tomé, Samuel
digcom.contributor.authorisAuthorOfPublication|email:xufei5056@gmail.com|institution:University of Massachusetts Amherst|Xu, Fei
digcom.contributor.authorisAuthorOfPublication|email:yanhuihan@foodsci.umass.edu|institution:University of Massachusetts Amherst|Han, Yanhui
digcom.contributor.authorisAuthorOfPublication|email:b.hernandez@csic.es|institution:Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM CEI UAM+CSIC)|Hernández-Ledesma, Bianca
digcom.contributor.authorisAuthorOfPublication|email:hangxiao@foodsci.umass.edu|institution:University of Massachusetts Amherst|Xiao, Hang
digcom.date.embargo2021-10-05T00:00:00-07:00
digcom.identifierfoodsci_faculty_pubs/36
digcom.identifier.contextkey25279633
digcom.identifier.submissionpathfoodsci_faculty_pubs/36
dspace.entity.typePublication
relation.isAuthorOfPublicationd278f0aa-07bb-422a-bcc6-db84a72bfe03
relation.isAuthorOfPublication.latestForDiscoveryd278f0aa-07bb-422a-bcc6-db84a72bfe03
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