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A multi-omics approach to unravelling the coupling mechanism of nitrogen metabolism and phenanthrene biodegradation in soil amended with biochar

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dc.contributoret. al.
dc.contributor.authorXing, Baoshan
dc.date2024-04-03T04:24:03.000
dc.date.accessioned2024-04-26T20:26:36Z
dc.date.available2024-04-26T20:26:36Z
dc.date.issued2024-01-01
dc.description.abstractThe presence of polycyclic aromatic hydrocarbons (PAHs) in soil negatively affects the environment and the degradation of these contaminants is influenced by nitrogen metabolism. However, the mechanisms underlying the interrelationships between the functional genes involved in nitrogen metabolism and phenanthrene (PHE) biodegradation, as well as the effects of biochar on these mechanisms, require further study. Therefore, this study utilised metabolomic and metagenomic analysis to investigate primary nitrogen processes, associated functional soil enzymes and functional genes, and differential soil metabolites in PHE-contaminated soil with and without biochar amendment over a 45-day incubation period. Results showed that dissimilatory nitrate reduction to ammonium (DNRA) and denitrification were the dominant nitrogen metabolism processes in PHE-contaminated soil. The addition of biochar enhanced nitrogen modules, exhibiting discernible temporal fluctuations in denitrification and DNRA proportions. Co-occurrence networks and correlation heatmap analysis revealed potential interactions among functional genes and enzymes responsible for PHE biodegradation and nitrogen metabolism. Notably, enzymes associated with denitrification and DNRA displayed significant positive correlation with enzymes involved in downstream phenanthrene degradation. Of particular interest was stronger correlation observed with the addition of biochar. However, biochar amendment inhibited the 9-phenanthrol degradation pathway, resulting in elevated levels of glutathione (GSH) in response to environmental stress. These findings provide new insights into the interactions between nitrogen metabolism and PHE biodegradation in soil and highlight the dual effects of biochar on these processes.
dc.identifier.doihttps://doi.org/10.1016/j.envint.2024.108435
dc.identifier.urihttps://hdl.handle.net/20.500.14394/43941
dc.relation.ispartofEnvironmental International
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1047&context=stockbridge_faculty_pubs&unstamped=1
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.issue183
dc.source.statuspublished
dc.subjectPHE biodegradation
dc.subjectNitrogen metabolism
dc.subjectCoupling
dc.subjectSoil differential metabolites
dc.titleA multi-omics approach to unravelling the coupling mechanism of nitrogen metabolism and phenanthrene biodegradation in soil amended with biochar
dc.typearticle
dc.typearticle
digcom.contributor.authorXing, Baoshan
digcom.identifierstockbridge_faculty_pubs/48
digcom.identifier.contextkey36819858
digcom.identifier.submissionpathstockbridge_faculty_pubs/48
dspace.entity.typePublication
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