Background: Nurses are constantly exposed to occupational and environmental issues with the potential to impact their health and safety. A relatively new technology with promising benefits for medicine, yet with potential health and environmental risks is Nanotechnology. Purpose: The aim of this project was to implement an educational program to increase nurses’ knowledge and awareness about Nanotechnology, its potential benefits and risks in healthcare. The single educational intervention was delivered in person, using PowerPoint presentation designed by the DNP student. The program provided definitions of Nanotechnology and Nanomedicine, it discussed nanoparticles and its application in medical and household products, addressed the benefits, potential health and safety risks, and nursing implications. Results: A pre- and posttest format design was utilized to examine the effect of the educational program on participants’ knowledge about Nanotechnology. The results from the knowledge surveys showed an increase of 77.1% in knowledge about Nanotechnology among the participants. Qualitative comments reflected the positive response towards the educational program. Conclusions: These findings have implications for nursing practice including providing educational opportunities for nurses so they are better equipped to identify medical products containing nanoparticles, to practice safely and to educate patients about treatments using such products. Keywords: nursing, Nanotechnology, Nanomedicine, nanoparticles, hazards exposures, healthcare, safety, and education.