In Vivo Investigations of Polymer Conjugates as Therapeutics

Henchey, Elizabeth M

Publication:
In Vivo Investigations of Polymer Conjugates as Therapeutics

dc.contributor.advisor	Sallie Smith Schneider
dc.contributor.author	Henchey, Elizabeth M
dc.contributor.department	University of Massachusetts Amherst
dc.contributor.department	Molecular & Cellular Biology
dc.date	2023-09-23T04:37:27.000
dc.date.accessioned	2024-04-26T21:13:45Z
dc.date.available	2011-08-31T00:00:00Z
dc.date.issued	2011-01-01
dc.date.submitted	September
dc.description.abstract	Polymer conjugates offer a way to introduce materials into the body that would normally be rejected or cause toxicity. Two polymers are investigated in vivo for uses in chemotherapeutic delivery, protein therapeutics, and DNA transfection. A novel polymer, polyMPC, has the ability to increase doxorubicin loading and its solubility, and is conjugated in a way to release its payload in a low pH environment. Through its conjugation, blood clearance time of doxorubicin is increased, and thus tumor exposure to the drug is increased with a single administration. It can be administered at ten times the concentration of free doxorubicin, and three times the concentration of Doxil®, while decreasing the cardio-toxicity normally associated with doxorubicin administration. These results show that polyMPC has the potential to increase treatment efficacy of doxorubicin. With increased circulation time, MPC polymers have additional potential for protein delivery and variations of its design were tested in linear, branched and grafted states, which show limited affect on tissue weight. An additional polymer for use in DNA transfection, NLS2, demonstrated its lack of tissue toxicity when injected intramuscularly. While continued investigation into these polymers is required, this initial data indicates their promising uses as therapeutics.
dc.description.degree	Master of Science (M.S.)
dc.identifier.doi	https://doi.org/10.7275/2208020
dc.identifier.uri	https://hdl.handle.net/20.500.14394/47594
dc.relation.url	https://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1826&context=theses&unstamped=1
dc.source.status	published
dc.subject	polymer
dc.subject	in vivo
dc.subject	breast cancer
dc.subject	Biochemistry
dc.subject	Biology
dc.subject	Cancer Biology
dc.subject	Laboratory and Basic Science Research
dc.subject	Molecular Biology
dc.subject	Other Animal Sciences
dc.title	In Vivo Investigations of Polymer Conjugates as Therapeutics
dc.type	open
dc.type	article
dc.type	thesis
digcom.contributor.author	isAuthorOfPublication\|email:ehenchey17@gmail.com\|institution:University of Massachusetts Amherst\|Henchey, Elizabeth M
digcom.date.embargo	2011-08-31T00:00:00-07:00
digcom.identifier	theses/684
digcom.identifier.contextkey	2208020
digcom.identifier.submissionpath	theses/684
dspace.entity.type	Publication