Publication:
Regulation of Trio Splice Variants by 17Β-Estradiol and 2,3,7,8-Tetrachlorodibenzo-p-dioxin

Simple item page
dc.contributor.advisorSandra L Petersen
dc.contributor.authorCunningham, Michael J
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentNeuroscience & Behavior
dc.date2023-09-22T20:32:50.000
dc.date.accessioned2024-04-26T21:09:05Z
dc.date.available2009-12-15T00:00:00Z
dc.date.issued2010-01-01
dc.date.submittedFebruary
dc.description.abstractThe anteroventral periventricular nucleus (AVPV) is a sexually dimorphic preoptic structure that is nearly three times larger in the female rat. Estradiol (E2) exposure during development decreases AVPV volume through apoptosis, a process which normally occurs preferentially in male. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is an endocrine disrupter that interferes with E2-dependent sexual differentiation of the AVPV. Whole-genome microarrays were used to identify sex-specific genes regulated by E2 and TCDD in postnatal day 2 (P2) AVPV punches from untreated males and females, males treated with TCDD or vehicle, and females treated with E2 or vehicle. Trio emerged as a gene target regulated by E2 and TCDD, and this gene is essential for neurite outgrowth which is sexually dimorphic in the AVPV. My goal was to verify microarray data that Trio was expressed in the P2 AVPV and to test whether expression was affected by sex, E2 and TCDD. The microarray did not discriminate among Trio splice variants, 9S, 9L and Duet, so I mapped each of these in the P2 AVPV using in situ hybridization. I used quantitative real-time PCR to examine the effects of sex, E2 and TCDD on mRNA levels encoding each splice variant. I found that the AVPV expressed 9S and 9L at moderate levels and Duet at low levels. The expression of 9S and 9L mRNA was increased by E2 in females. However, only 9S expression differed between sexes and it was lower in males. TCDD had no effect on expression of any of the splice variants. This work provides the first evidence that Trio splice variants are independently regulated and that Trio may mediate effects of E2 in the developing AVPV.
dc.description.degreeMaster of Science (M.S.)
dc.identifier.doihttps://doi.org/10.7275/1089301
dc.identifier.urihttps://hdl.handle.net/20.500.14394/47282
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1484&context=theses&unstamped=1
dc.source.statuspublished
dc.subjectMolecular biology
dc.subjectTrio
dc.subjectAVPV
dc.subjectTCDD
dc.subjectsexual dimorphism
dc.subjectsplice variants
dc.subjectBiology
dc.subjectMolecular and Cellular Neuroscience
dc.subjectGenetics
dc.titleRegulation of Trio Splice Variants by 17Β-Estradiol and 2,3,7,8-Tetrachlorodibenzo-p-dioxin
dc.typecampus
dc.typearticle
dc.typethesis
digcom.contributor.authorisAuthorOfPublication|email:mcunning22@gmail.com|institution:University of Massachusetts Amherst|Cunningham, Michael J
digcom.date.embargo2009-12-15T00:00:00-08:00
digcom.identifiertheses/400
digcom.identifier.contextkey1089301
digcom.identifier.submissionpaththeses/400
dspace.entity.typePublication
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Final_Revised._1.21.10.Thesis.Final.pdf
Size:
786.23 KB
Format:
Adobe Portable Document Format
Name: Final_Revised._1.21.10.Thesis.Final.pdf
Size: 786.23 KB
Kind: Adobe PDF
Collections
Masters Theses