The purpose of this study is to investigate what semiotic analysis can reveal about how welcoming and inclusive a science library space is for patrons. A space was examined in terms of its messaging to patrons of various genders, abilities, races, ethnicities, nationalities, and disciplines. Findings are presented about the space’s messaging regarding the disciplines it serves, inclusion of patrons from minoritized demographics, and general expectations of visitors. Recommendations for library practice and future research are discussed.