The role of bacterial microcompartments in the fermentation of D-arabinose in Clostridium phytofermentans

Strough, Megan A

Publication:
The role of bacterial microcompartments in the fermentation of D-arabinose in Clostridium phytofermentans

dc.contributor.advisor	Jeffrey L. Blanchard
dc.contributor.advisor	Susan B. Leschine
dc.contributor.author	Strough, Megan A
dc.contributor.department	University of Massachusetts Amherst
dc.contributor.department	Microbiology
dc.date	2023-09-23T07:51:36.000
dc.date.accessioned	2024-04-26T20:36:41Z
dc.date.available	2013-04-11T00:00:00Z
dc.date.issued	2013-01-01
dc.date.submitted	May
dc.description.abstract	Bacterial microcompartments, or BMCs, are 80-200nm, enzyme-encompassing organelles composed of interlocking proteins that form cyclical hexamers with a small central pore. Clostridium phytofermentans or Cphy, is a Gram-positive, rod shaped, anaerobic soil microbe that has the ability to not only break down multiple polysaccharides simultaneously but also proceeds to ferment them into biofuels. The genome of Cphy contains 3 BMC loci. During growth on fucose and rhamnose, one of these loci is highly expressed and microcompartments can be viewed using TEM. Under these growth conditions, three products, ethanol, propanol and propionate, which could potentially be highly useful in the biofuel and bioproducts industries, are produced. Gene expression microarrays have revealed that the genes for the fucose/rhamnose-related microcompartment are also highly expressed on D-arabinose. The role of BMCs during growth on D-arabinose has not been reported in any organism. My goal is to determine the role of BMCs in D-arabinose metabolism in C. phytofermentans.
dc.description.degree	Master of Science (M.S.)
dc.identifier.doi	https://doi.org/10.7275/4025495
dc.identifier.uri	https://hdl.handle.net/20.500.14394/44521
dc.relation.url	https://scholarworks.umass.edu/cgi/viewcontent.cgi?article=2162&context=theses&unstamped=1
dc.source.status	published
dc.subject	Bacteriology
dc.subject	Bioinformatics
dc.subject	Environmental Microbiology and Microbial Ecology
dc.subject	Genomics
dc.subject	Microbial Physiology
dc.subject	Molecular Genetics
dc.title	The role of bacterial microcompartments in the fermentation of D-arabinose in Clostridium phytofermentans
dc.type	open
dc.type	article
dc.type	thesis
digcom.contributor.author	isAuthorOfPublication\|email:mstrough@cns.umass.edu\|institution:University of Massachusetts Amherst\|Strough, Megan A
digcom.date.embargo	2013-04-11T00:00:00-07:00
digcom.identifier	theses/1088
digcom.identifier.contextkey	4025495
digcom.identifier.submissionpath	theses/1088
dspace.entity.type	Publication