Publication:
Cryptic Materials And Coacervates

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dc.contributor.advisorSarah L. Perry
dc.contributor.advisorJohn Klier
dc.contributor.authorSun, Yimin
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentChemical Engineering
dc.date2024-03-28T20:20:25.000
dc.date.accessioned2024-04-26T18:08:25Z
dc.date.available2024-04-26T18:08:25Z
dc.date.issued2021-05-14
dc.date.submittedMay
dc.date.submitted2021
dc.description.abstractHydrogels have been used for many applications, including as a mimic for the extracellular matrix (ECM) in cell culture. For example, a hydrogel containing protease-sensitive substrates can be used to create an environment that cells can modify via enzymatic degradation. In this study, we propose combining traditional hydrogels for cell culture with “cryptic” site that bury proteolytically cleavable peptide sequences using complex coacervation. Here, the goal is to take advantage of the phase separation of coacervates to protect the cleavable peptide against degradation until acted upon by a mechanical force, such as those generated by adherent cells. To this end, we studied the encapsulation of chymotrypsin as a model protease into our coacervate system and investigated the effect of incorporation into the coacervate on its activity. We have also synthesized a peptide containing cleavable site for both chymotrypsin as our model protease and more biologically relevant matrix metalloproteinases (MMPs). Future efforts will look to incorporate this peptide into both coacervate and hydrogel and test the level of cryptic response.
dc.description.degreeMaster of Science in Chemical Engineering (M.S.Ch.E.)
dc.identifier.doihttps://doi.org/10.7275/23585029.0
dc.identifier.orcidhttps://orcid.org/0000-0001-6222-2571
dc.identifier.urihttps://hdl.handle.net/20.500.14394/32746
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=2138&context=masters_theses_2&unstamped=1
dc.source.statuspublished
dc.subjectCoacervate
dc.subjectChymotrypsin
dc.subjectPeptide
dc.titleCryptic Materials And Coacervates
dc.typecampusfive
dc.typearticle
dc.typethesis
digcom.contributor.authorisAuthorOfPublication|email:17720818713@sina.cn|institution:University of Massachusetts Amherst|Sun, Yimin
digcom.identifiermasters_theses_2/1083
digcom.identifier.contextkey23585029
digcom.identifier.submissionpathmasters_theses_2/1083
dspace.entity.typePublication
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