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Click Chemistry Approach to Analyze Curcumin-Protein Interactions in vitro and in vivo

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dc.contributor.advisorGuodong Zhang
dc.contributor.authorZhou, Jingyi
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentFood Science
dc.date2023-09-23T23:09:18.000
dc.date.accessioned2024-04-26T18:27:16Z
dc.date.available2024-04-26T18:27:16Z
dc.date.submittedMay
dc.date.submitted2019
dc.description.abstractOver the past decades, numerous studies shown curcumin, a dietary compound derived from turmeric, has a variety of health-promoting actions, such as anti-oxidant, anti-microbial, anti-inflammatory, and anti-cancer effects, making curcumin the most promising dietary compound for disease prevention. However, the underlying mechanisms by which curcumin has these health-promoting effects are not well understood. A better understanding of the molecular mechanism of curcumin could help to develop novel strategies to reduce the risks of some human diseases. Protein thiols play important roles in cell signaling, and recent studies showed that curcumin could covalently react with protein thiols, supporting that curcumin-protein interactions could contribute to the health-promoting effects of curcumin. However, the curcumin-protein interactions are under-studied. Notably, it remains unknown whether oral intake of curcumin could covalently interact with protein in vivo. In this project, we synthesized a click chemistry probe of curcumin (Di-Cur), and used this probe to characterize curcumin-protein interactions both in vitro and in vivo using a click chemistry-based imaging approach. Our results demonstrate that orally administrated curcumin could form curcumin-protein adducts in specific tissues of the mice, which may contribute to the potent biological effects and poor pharmacokinetics of curcumin.
dc.description.degreeMaster of Science (M.S.)
dc.identifier.doihttps://doi.org/10.7275/14188070
dc.identifier.orcidhttps://orcid.org/0000-0002-7020-598X
dc.identifier.urihttps://hdl.handle.net/20.500.14394/33866
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1811&context=masters_theses_2&unstamped=1
dc.source.statuspublished
dc.subjectCurcumin
dc.subjectDi-Cur
dc.subjectProtein
dc.subjectClick Chemistry
dc.subjectOther Food Science
dc.titleClick Chemistry Approach to Analyze Curcumin-Protein Interactions in vitro and in vivo
dc.typecampusfive
dc.typearticle
dc.typethesis
digcom.contributor.authorisAuthorOfPublication|email:jingyizhou@umass.edu|institution:University of Massachusetts Amherst|Zhou, Jingyi
digcom.identifiermasters_theses_2/810
digcom.identifier.contextkey14188070
digcom.identifier.submissionpathmasters_theses_2/810
dspace.entity.typePublication
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