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RNAi Validation of Resistance Genes and Their Interactions in the Highly DDT-Resistant 91-R Strain of Drosophila Melanogaster

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dc.contributor.advisorJohn M Clark
dc.contributor.advisorJennifer Normanly
dc.contributor.advisorKathleen Arcaro
dc.contributor.authorGellatly, Kyle
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentMolecular & Cellular Biology
dc.date2024-03-28T19:59:30.000
dc.date.accessioned2024-04-26T18:17:30Z
dc.date.available2024-04-26T18:17:30Z
dc.date.submittedSeptember
dc.date.submitted2015
dc.description.abstract4,4’-dichlorodiphenyltrichloroethane (DDT) has been re-recommended by the World Health Organization for malaria mosquito control. Previous DDT use has resulted in resistance, and with continued use resistance will increase in terms of level and extent. Drosophila melanogaster is a model dipteran that has many available genetic tools, numerous studies done on insecticide resistance mechanisms, and is related to malaria mosquitoes allowing for extrapolation. The 91-R strain of D. melanogaster is highly resistant to DDT (>1500-fold), however, there is no mechanistic scheme that accounts for this level of resistance. Recently, reduced penetration, increased detoxification, and direct excretion have been identified as resistance mechanisms in the 91-R strain. Their interactions, however, remain unclear. Use of UAS-RNAi transgenic lines of D. melanogaster allowed for the targeted knockdown of genes putatively involved in DDT resistance and has validated the role of several cuticular proteins (Cyp4g1 and Lcp1), cytochrome P450 monooxygenases (Cyp6g1 and Cyp12d1), and ATP binding cassette transporters (Mdr50, Mdr65, and Mrp1) involved in DDT resistance.
dc.description.degreeMaster of Science (M.S.)
dc.identifier.doihttps://doi.org/10.7275/7335178
dc.identifier.orcidN/A
dc.identifier.urihttps://hdl.handle.net/20.500.14394/33267
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1286&context=masters_theses_2&unstamped=1
dc.source.statuspublished
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.titleRNAi Validation of Resistance Genes and Their Interactions in the Highly DDT-Resistant 91-R Strain of Drosophila Melanogaster
dc.typeopenaccess
dc.typearticle
dc.typethesis
digcom.contributor.authorisAuthorOfPublication|email:kgellatl@student.umass.edu|institution:University of Massachusetts Amherst|Gellatly, Kyle
digcom.identifiermasters_theses_2/271
digcom.identifier.contextkey7335178
digcom.identifier.submissionpathmasters_theses_2/271
dspace.entity.typePublication
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