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Microtubule plus-ends act as physical signaling hubs to activate RhoA during cytokinesis

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dc.contributor.authorVerma, Vikash
dc.contributor.authorMaresca, Thomas J.
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.date2023-09-23T23:15:11.000
dc.date.accessioned2024-04-26T08:41:22Z
dc.date.available2019-03-22T00:00:00Z
dc.date.issued2019-01-01
dc.description.abstractMicrotubules (MTs) are essential for cleavage furrow positioning during cytokinesis, but the mechanisms by which MT-derived signals spatially define regions of cortical contractility are unresolved. In this study cytokinesis regulators visualized in Drosophila melanogaster (Dm) cells were found to localize to and track MT plus-ends during cytokinesis. The RhoA GEF Pebble (Dm ECT2) did not evidently tip-track, but rather localized rapidly to cortical sites contacted by MT plus-tips, resulting in RhoA activation and enrichment of myosin-regulatory light chain. The MT plus-end localization of centralspindlin was compromised following EB1 depletion, which resulted in a higher incidence of cytokinesis failure. Centralspindlin plus-tip localization depended on the C-terminus and a putative EB1-interaction motif (hxxPTxh) in RacGAP50C. We propose that MT plus-end-associated centralspindlin recruits a cortical pool of Dm ECT2 upon physical contact to activate RhoA and to trigger localized contractility.
dc.identifier.doihttps://doi.org/10.7554/eLife.38968.001
dc.identifier.urihttps://hdl.handle.net/20.500.14394/4309
dc.relation.ispartofeLife
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1634&context=biology_faculty_pubs&unstamped=1
dc.rightsUMass Amherst Open Access Policy
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.issue8
dc.source.statuspublished
dc.subjectBiology
dc.titleMicrotubule plus-ends act as physical signaling hubs to activate RhoA during cytokinesis
dc.typearticle
dc.typearticle
digcom.contributor.authorVerma, Vikash
digcom.contributor.authorisAuthorOfPublication|email:tmaresca@bio.umass.edu|institution:University of Massachusetts Amherst|Maresca, Thomas J.
digcom.date.embargo2019-03-22T00:00:00-07:00
digcom.identifierbiology_faculty_pubs/635
digcom.identifier.contextkey14085394
digcom.identifier.submissionpathbiology_faculty_pubs/635
dspace.entity.typePublication
relation.isAuthorOfPublication9c6c472f-8bd3-41dd-9917-8b7f9a715899
relation.isAuthorOfPublication.latestForDiscovery9c6c472f-8bd3-41dd-9917-8b7f9a715899
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