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<em>Bifidobacterium infantis</em> utilizes N-acetylglucosamine-containing human milk oligosaccharides as a nitrogen source

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dc.contributor.authorLi, Shuqi
dc.contributor.authorYou, Xiaomeng
dc.contributor.authorRani, Asha
dc.contributor.authorÖzcan, Ezgi
dc.contributor.authorSela, David A
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.date2023-09-24T10:12:40.000
dc.date.accessioned2024-04-26T17:31:40Z
dc.date.available2023-09-14T00:00:00Z
dc.date.issued2023-01-01
dc.description.abstractBifidobacterium longum subsp. infantis (B. infantis) utilizes oligosaccharides secreted in human milk as a carbohydrate source. These human milk oligosaccharides (HMOs) integrate the nitrogenous residue N-acetylglucosamine (NAG), although HMO nitrogen utilization has not been described to date. Herein, we characterize the B. infantis nitrogen utilization phenotype on two NAG-containing HMO species, LNT and LNnT. This was characterized through in vitro growth kinetics, incorporation of isotopically labeled NAG nitrogen into the proteome, as well as modulation of intracellular 2-oxoglutarate levels while utilizing HMO nitrogen. Further support is provided by comparative transcriptomics and proteomics that identified global regulatory networks deployed during HMO nitrogen utilization. The aggregate data demonstrate that B. infantis strains utilize HMO nitrogen with the potential to significantly impact fundamental and clinical studies, as well as enable applications.
dc.identifier.doihttps://doi.org/10.1080/19490976.2023.2244721
dc.identifier.orcidLi: https://orcid.org/0000-0002-7626-4606, You: https://orcid.org/0000-0003-2132-1404, Rani: https://orcid.org/0000-0002-8556-4715, Özcan: https://orcid.org/0000-0001-5365-1550, Sela: https://orcid.org/0000-0002-3354-7470
dc.identifier.urihttps://hdl.handle.net/20.500.14394/29632
dc.relation.ispartofGut Microbes
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1073&amp;context=foodsci_faculty_pubs&amp;unstamped=1
dc.rightsUMass Amherst Open Access Policy
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.issue2
dc.source.issue15
dc.source.statuspublished
dc.subjecthuman milk oligosaccharides
dc.subjectmicrobiota
dc.subjectbifidobacteria
dc.subjectnitrogen metabolism
dc.subject2-oxoglutarate
dc.title<em>Bifidobacterium infantis</em> utilizes N-acetylglucosamine-containing human milk oligosaccharides as a nitrogen source
dc.typearticle
dc.typearticle
digcom.contributor.authorisAuthorOfPublication|email:shuqi.li65@gmail.com|institution:University of Massachusetts Amherst|Li, Shuqi
digcom.contributor.authorisAuthorOfPublication|email:youxiaomeng1003@gmail.com|institution:University of Massachusetts Amherst|You, Xiaomeng
digcom.contributor.authorRani, Asha
digcom.contributor.authorisAuthorOfPublication|email:eozcan@umass.edu|institution:University of Massachusetts Amherst|Özcan, Ezgi
digcom.contributor.authorisAuthorOfPublication|email:davidsela@umass.edu|institution:University of Massachusetts Amherst|Sela, David A
digcom.date.embargo2023-09-14T00:00:00-07:00
digcom.identifierfoodsci_faculty_pubs/73
digcom.identifier.contextkey36044785
digcom.identifier.submissionpathfoodsci_faculty_pubs/73
dspace.entity.typePublication
relation.isAuthorOfPublication46d5d02f-51c4-436d-ab3e-7c6790a55fef
relation.isAuthorOfPublication.latestForDiscovery46d5d02f-51c4-436d-ab3e-7c6790a55fef
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