Publication:
Intracellular Membrane Organization in Mycobacteria

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dc.contributor.advisorYasu S. Morita
dc.contributor.advisorMichele M. Klingbeil
dc.contributor.advisorPeter Chien
dc.contributor.advisorSteven J. Sandler
dc.contributor.authorHayashi, Jennifer
dc.contributor.departmentUniversity of Massachusetts Amherst
dc.date2023-09-23 16:45:09
dc.date.accessioned2024-04-26T16:18:02Z
dc.date.available2024-04-26T16:18:02Z
dc.date.issued2017
dc.date.submittedFebruary
dc.date.submitted2017
dc.description.abstractMycobacterium is a diverse genus of actinobacteria that includes the causative agents of human tuberculosis and leprosy. Mycobacteria are protected by their unique, multilaminar cell envelope, which grants them intrinsic resistance to environmental challenges such as antibiotics. This essential cellular structure is elongated at the polar ends of cells, but the regulation of cytosolic precursor synthesis and localized envelope synthesis remains unclear. Here, we present the PMf (plasma membrane free of cell wall components), a membrane domain distinct from the bulk plasma membrane of Mycobacterium smegmatis. Proteomic and lipidomic characterization demonstrate that the PMf contains lipidic substrates of cell envelope synthesis as well as the essential enzymes that produce these lipids. Live imaging of cells expressing PMf-targeted fluorescent reporters reveals that the PMf is enriched near the site of new cell envelope synthesis and is present upon the formation of a new growth pole. Additionally, we found that during nutrient starvation and antibiotic treatment, the PMf redistributes from the cell poles to the sidewall of the cell body, suggesting its role in general cell envelope maintenance. These observations support a model in which the PMf functions to spatiotemporally coordinate cell elongation by providing the necessary precursor molecules at the site of envelope synthesis.
dc.description.degreeDoctor of Philosophy (PhD)
dc.description.departmentMicrobiology
dc.identifier.doihttps://doi.org/10.7275/9473986.0
dc.identifier.orcidN/A
dc.identifier.urihttps://hdl.handle.net/20.500.14394/20138
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1927&context=dissertations_2&unstamped=1
dc.source.statuspublished
dc.subjectMycobacterium
dc.subjectMembrane domain
dc.subjectCell envelope
dc.subjectGrowth
dc.subjectMicrobiology
dc.titleIntracellular Membrane Organization in Mycobacteria
dc.typeopenaccess
dc.typedissertation
digcom.contributor.authorisAuthorOfPublication|email:jhayashi@cns.umass.edu|institution:University of Massachusetts Amherst|Hayashi, Jennifer
digcom.identifierdissertations_2/877
digcom.identifier.contextkey9473986
digcom.identifier.submissionpathdissertations_2/877
dspace.entity.typePublication
relation.isAuthorOfPublicationf554831d-1021-4de1-b562-266020cd13a6
relation.isAuthorOfPublication.latestForDiscoveryf554831d-1021-4de1-b562-266020cd13a6
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