Publication:
Identification And Functional Characterization Of Zebrafish Gene Technotrousers (tnt)

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dc.contributor.advisorGerald B. Downes
dc.contributor.advisorRichard E. van Emmerik
dc.contributor.advisorAbigail M. Jensen
dc.contributor.authorMcKeown, Kelly Anne
dc.contributor.departmentUniversity of Massachusetts - Amherst
dc.date2023-09-23T09:14:14.000
dc.date.accessioned2024-04-26T13:51:17Z
dc.date.available2014-06-10T00:00:00Z
dc.date.issued2010-09-01
dc.description.abstractNeural networks in the hindbrain and spinal cord require a proper balance between excitation and inhibition. Identification of zebrafish mutants that have defects in motor output mediated by these networks can allow entrée into underlying network mechanisms. techno trouser ( tnt ) mutants demonstrate abnormal motor behavior. Two days after fertilization, wild-type larvae perform an escape response consisting of a large-amplitude body bend away from touch stimuli followed by smaller amplitude body bends to swim away. tntmutants perform an initial large amplitude body bend away from touch stimuli, but the following smaller amplitude body bends are interrupted by several, abnormal, large amplitude body bends. Four days after fertilization, wild-type larvae exhibit faster escape behavior, whereas tnt mutants are nearly paralyzed and shorter along the rostral-caudal axis. We used meiotic mapping and candidate gene analysis to reveal that thetnt mutation disrupts slc1a2b , which encodes EAAT2, a glutamate transporter expressed in glial cells. Lesion analysis, in situ hybridization, and in vivo electrophysiological recordings all support a model in which reducedslc1a2b function results in exuberant excitation of neurons, initially in the hindbrain and later in development in the spinal cord, to produce the large-amplitude body bends and subsequent paralysis of tnt mutants. Since disruption of human EAAT2 is thought to promote several different neurological diseases, including epilepsy and amyotrophic lateral sclerosis, tnt mutants provide a new tool to understand these disorders.
dc.description.degreeDoctor of Philosophy (PhD)
dc.description.departmentMolecular and Cellular Biology
dc.identifier.doihttps://doi.org/10.7275/5674950
dc.identifier.urihttps://hdl.handle.net/20.500.14394/12119
dc.relation.urlhttps://scholarworks.umass.edu/cgi/viewcontent.cgi?article=1203&context=dissertations_1&unstamped=1
dc.source.statuspublished
dc.subjectBiological sciences
dc.subjectBehavior
dc.subjectGlial glutamate transporter
dc.subjectLocomotion
dc.subjectMotor develoment
dc.subjectZebrafish
dc.subjectTechnotrousers
dc.subjectCell Biology
dc.subjectMolecular Biology
dc.subjectNeuroscience and Neurobiology
dc.titleIdentification And Functional Characterization Of Zebrafish Gene Technotrousers (tnt)
dc.typecampus
dc.typearticle
dc.typedissertation
digcom.contributor.authorMcKeown, Kelly Anne
digcom.date.embargo2014-06-10T00:00:00-07:00
digcom.identifierdissertations_1/204
digcom.identifier.contextkey5674950
digcom.identifier.submissionpathdissertations_1/204
dspace.entity.typePublication
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