The last six years has seen a remarkable transformation in our collective ability to deliver sustainable outcomes at the landscape and watershed level, especially for aquatic organism passage (AOP). While command and control regulatory mechanisms continue to play a key role in the United States, significant advances have been made in using collaborative and coordinated investments in infrastructure and conservation to meet multiple goals. The Federal Highway Administration, for example, funded development and deployment of a synthesis for AOP; and internationally significant Hydraulic Engineering Circular on AOP through culverts (HEC 26); and engineering guidelines for environmentally sound instream countermeasures is underway. Effective collaboration and coordinating investments in infrastructure and conservation has been deployed under the Ecological Approach, Livability, and Transitioning to Sustainability initiatives. This paper describes how HEC 26 is intended to be used as a decision support tool for landscape or watershed scale sustainability initiatives.