Publication Date
2023
Journal or Book Title
Gut Microbes
Abstract
Bifidobacterium longum subsp. infantis (B. infantis) utilizes oligosaccharides secreted in human milk as a carbohydrate source. These human milk oligosaccharides (HMOs) integrate the nitrogenous residue N-acetylglucosamine (NAG), although HMO nitrogen utilization has not been described to date. Herein, we characterize the B. infantis nitrogen utilization phenotype on two NAG-containing HMO species, LNT and LNnT. This was characterized through in vitro growth kinetics, incorporation of isotopically labeled NAG nitrogen into the proteome, as well as modulation of intracellular 2-oxoglutarate levels while utilizing HMO nitrogen. Further support is provided by comparative transcriptomics and proteomics that identified global regulatory networks deployed during HMO nitrogen utilization. The aggregate data demonstrate that B. infantis strains utilize HMO nitrogen with the potential to significantly impact fundamental and clinical studies, as well as enable applications.
ORCID
Li: https://orcid.org/0000-0002-7626-4606, You: https://orcid.org/0000-0003-2132-1404, Rani: https://orcid.org/0000-0002-8556-4715, Özcan: https://orcid.org/0000-0001-5365-1550, Sela: https://orcid.org/0000-0002-3354-7470
DOI
https://doi.org/10.1080/19490976.2023.2244721
Volume
15
Issue
2
License
UMass Amherst Open Access Policy
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Li, Shuqi; You, Xiaomeng; Rani, Asha; Özcan, Ezgi; and Sela, David A., "Bifidobacterium infantis utilizes N-acetylglucosamine-containing human milk oligosaccharides as a nitrogen source" (2023). Gut Microbes. 73.
https://doi.org/10.1080/19490976.2023.2244721