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Open Access Thesis
Molecular & Cellular Biology
Master of Science (M.S.)
Year Degree Awarded
Month Degree Awarded
The degradation and recycling of protein is a process essential for the maintenance and regulation of cellular function. More specifically, in Caulobacter crescentus, the ClpXP protease is responsible for driving progression through the cell cycle and protein quality control. This protease utilizes three known adaptors to selectively degrade proteins that initiate different stages of development. This thesis will elaborate on the specific binding interface on one of these adaptors, PopA, with another, RcdA, and focus in on specific residues on PopA and investigate their roles in adaptor binding and delivery of CtrA, the master regulator of Caulobacter. Finally, I will investigate the relationship between and necessity of these adaptors using a mutant PopA that does not require the presence of RcdA or the other adaptor, CpdR. The remainder of this thesis will present data that arises from these projects.
Scudder, Thomas P., "Caulobacter ClpXP Adaptor PopA’s Domain Interactions in the Adaptor Hierarchy of CtrA Degradation" (2023). Masters Theses. 1344.