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ORCID
https://orcid.org/0009-0008-5241-3356
Access Type
Open Access Thesis
Document Type
thesis
Degree Program
Animal Science
Degree Type
Master of Science (M.S.)
Year Degree Awarded
2023
Month Degree Awarded
September
Abstract
Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identification of subcellular localization and characterization of the function of target proteins of interest. However, because there can be considerable sequence diversity between orthologous proteins in Xenopus and mammals, antibodies produced against mouse or human proteins often do not recognize Xenopus counterparts. To address this issue, we refined existing mouse monoclonal antibody production protocols to generate antibodies against Xenopus proteins of interest. Here, we describe several approaches for the generation of useful mouse anti-Xenopus antibodies to multiple Xenopus proteins and their validation in various experimental approaches. These novel antibodies are now available to the research community through the Developmental Study Hybridoma Bank (DSHB).
DOI
https://doi.org/10.7275/36024966.0
First Advisor
Dominique Alfandari
Second Advisor
Helene Cousin
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Horr, Brett A., "Production and Characterization of Monoclonal Antibodies to Xenopus Proteins" (2023). Masters Theses. 1383.
https://doi.org/10.7275/36024966.0
https://scholarworks.umass.edu/masters_theses_2/1383